Journal: Neuroscience Bulletin

Article Title: HOCPCA Exerts Neuroprotection on Retinal Ganglion Cells by Binding to CaMKIIα and Modulating Oxidative Stress and Neuroinflammation in Experimental Glaucoma

doi: 10.1007/s12264-025-01417-0

Figure Lengend Snippet: A Representative images of immunofluorescence labeling of CaMKIIα, β, and γ isoforms, and CaMKIV in retinal cryosections after Pressure exposure and HOCPCA treatment. White arrows, CaMK-positive cells; scale bar, 50 μm. B Quantification of CaMKIIα+, CaMKIIβ+, CaMKIIγ+, and CaMKIV+ cells in the GCL of retinas treated with HOCPCA after Pressure. C Representative co-labeling of CaMKIIα and CaMKIIβ isoforms in retinal cryosections after Pressure and HOCPCA treatment. White arrows, CaMKIIα+CaMKIIβ+ cells; scale bar, 50 μm. D The original images shown in the rectangular panel from ( C ) are overlaid with illustrations and intensity profiles measured along vectors across two fluorescent structures, confirming the colocalization of CaMKIIα and β. Scale bar, 10 μm. E Quantification of CaMKIIα+ CaMKIIβ+ cells in the GCL after HOCPCA treatment under pressure. F Composition of CaMKIIα+ cells (CaMKIIα+ CaMKIIβ+ cells and single CaMKIIα+ cells) in the GCL. Data are presented as the mean ± SEM ( n = 6 per group, * P < 0.05, ** P <0.01, *** P <0.001, **** P <0.0001, one-way ANOVA with Tukey's multiple comparisons test).

Article Snippet: CaMKIIγ , 1:1000 , Proteintech, #12666-2-AP.

Techniques: Immunofluorescence, Labeling

Journal: Neuroscience Bulletin

Article Title: HOCPCA Exerts Neuroprotection on Retinal Ganglion Cells by Binding to CaMKIIα and Modulating Oxidative Stress and Neuroinflammation in Experimental Glaucoma

doi: 10.1007/s12264-025-01417-0

Figure Lengend Snippet: A Representative images of immunofluorescence labeling of CaMKIIα, β, and γ isoforms, and CaMKIV in retinal cryosections after Pressure exposure and HOCPCA treatment. White arrows, CaMK-positive cells; scale bar, 50 μm. B Quantification of CaMKIIα+, CaMKIIβ+, CaMKIIγ+, and CaMKIV+ cells in the GCL of retinas treated with HOCPCA after Pressure. C Representative co-labeling of CaMKIIα and CaMKIIβ isoforms in retinal cryosections after Pressure and HOCPCA treatment. White arrows, CaMKIIα+CaMKIIβ+ cells; scale bar, 50 μm. D The original images shown in the rectangular panel from ( C ) are overlaid with illustrations and intensity profiles measured along vectors across two fluorescent structures, confirming the colocalization of CaMKIIα and β. Scale bar, 10 μm. E Quantification of CaMKIIα+ CaMKIIβ+ cells in the GCL after HOCPCA treatment under pressure. F Composition of CaMKIIα+ cells (CaMKIIα+ CaMKIIβ+ cells and single CaMKIIα+ cells) in the GCL. Data are presented as the mean ± SEM ( n = 6 per group, * P < 0.05, ** P <0.01, *** P <0.001, **** P <0.0001, one-way ANOVA with Tukey's multiple comparisons test).

Article Snippet: CaMKIIγ (rabbit) , Proteintech, #12666-2-AP , 1:200 , GαRb A594, 1:1000, Invitrogen.

Techniques: Immunofluorescence, Labeling